pG6L6 CAT (P#1541)
(Plasmid #14590)

• Depositing Lab: Matija Peterlin
• Publication: Zhang et al Genes Dev. 2003 Mar 15. 17(6):748-58.

Backbone

• Vector backbone: HIVSCAT-> HIVLTR linked to CAT reporter
• Backbone size w/o insert (bp): 4200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 5xGal4 and 5xLexA binding sites

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The reporter construct pG6L6CAT was made by inserting two oligonucleotides into the plasmid reporter pHIVSCAT (Lu et al. 1993, J Virol 67:1752-1760). The first one contained five modified LexA operator sequences (5'-GTACTGTATGTACATACAGTAC-3') and was inserted into the XmaI site, whereas the second one with five modified Gal4 DNA-binding sequences (5'-CGGAGTACT GTCCTCCGAG-3') was inserted into the KpnI site, which is located 442 bp upstream of LexA operator sites. See author's map for more information.