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pEF-HA-NBP1 (P#1261)
(Plasmid #14586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
  • Alt name
    NBP1, VMA13; CGI-11; MSTP042; SFDbeta; SFDalpha
  • Alt name
    Nef binding protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    ATP6V1H (a.k.a. CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer EF1a forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The coding region of NBP1 was amplified by PCR using primers containing the XbaI site and the HA tag at the 5' end and the ClaI site at the 3' end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-HA-NBP1 (P#1261) was a gift from Matija Peterlin (Addgene plasmid # 14586 ; http://n2t.net/addgene:14586 ; RRID:Addgene_14586)

  • For your References section:

    Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4. Lu X, Yu H, Liu SH, Brodsky FM, Peterlin BM. Immunity. 1998 May . 8(5):647-56. 10.1016/S1074-7613(00)80569-5 PubMed 9620685
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