pcDNA3.1 Flag Runx1 FL (P#1790)
(Plasmid #14585)

• Depositing Lab: Matija Peterlin
• Publication: Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-83.

Backbone

• Vector backbone: pcDNA3.1 Hygro
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Runx1
• Species: M. musculus (mouse)
• Insert Size (bp): 1353
• Entrez Gene: Runx1 (a.k.a. AML1, CBF-alpha-2, Cbfa2, Pebp2a2, Pebpa2b)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Runx1 insert in this plasmid has an extra 34 amino acids appended onto the c-terminus. The depositor states that this does not affect plasmid function and the plasmid should behave according to the associated publication.

Using PCR cloning, full-length Runx1 (1-451) was cloned into the HindIII/XhoI sites of pcDNA3.1Hygro. 5' primer has Kozak and Flag epitope-tag sequences. The plasmid was used in western blot and coimmunoprecipitation.