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PurposeLentiviral vector genome to ectopically express human TMPRSS2 variant 1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 145843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRRL.sin.cPPT.SFFV/IRES-neo.WPRE.
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman TMPRSS2 variant 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1590
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MutationG8V
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Entrez GeneTMPRSS2 (a.k.a. PRSS10)
- Promoter SFFV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bylentiviral puroR: puroR replaced by neoR RRL.sin.cPPT.SFFV/IRES-neo.WPRE
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NM_001135099 with 1 mutation G8V
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130) was a gift from Caroline Goujon (Addgene plasmid # 145843 ; http://n2t.net/addgene:145843 ; RRID:Addgene_145843) -
For your References section:
SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. Rebendenne A, Valadao ALC, Tauziet M, Maarifi G, Bonaventure B, McKellar J, Planes R, Nisole S, Arnaud-Arnould M, Moncorge O, Goujon C. J Virol. 2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20. 10.1128/JVI.02415-20 PubMed 33514628