pAID5.3-N
(Plasmid
#145812)
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Purpose(Empty Backbone) An all-in-one bicistronic plasmid for controlling protein degradation by AID technology
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 145812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepT2AL200R175-CAGGS-EGFP
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Vector typeMammalian Expression
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- mAID (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAID5.3-N was a gift from Masato Kanemaki (Addgene plasmid # 145812 ; http://n2t.net/addgene:145812 ; RRID:Addgene_145812) -
For your References section:
Constructing Auxin-Inducible Degron Mutants Using an All-in-One Vector. Yesbolatova A, Saito Y, Kanemaki MT. Pharmaceuticals (Basel). 2020 May 23;13(5). pii: ph13050103. doi: 10.3390/ph13050103. 10.3390/ph13050103 PubMed 32456235
