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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUPER
- Backbone size w/o insert (bp) 3176
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATM shRNA
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Alt nameATM
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SpeciesH. sapiens (human)
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Insert Size (bp)60
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Entrez GeneATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sense oligo sequence used for cloning: ATM, 5'-GATCCCCGGA TTTGCGTATT ACTCAGTTCA AGAGACTGAG TAATACGCAA ATCCTTTTTG GAAA-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUPER.ATMi was a gift from Didier Trono (Addgene plasmid # 14581 ; http://n2t.net/addgene:14581 ; RRID:Addgene_14581) -
For your References section:
DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi Y, Turelli P, Masutani M, Trono D. J Virol. 2005 Mar . 79(5):2973-8. 10.1128/JVI.79.5.2973-2978.2005 PubMed 15709017