pAtetO_6xHis-Bst-LF
(Plasmid #145799)

• Purpose: Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP
• Depositing Lab: Andrew Ellington
• Publication: Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi: 10.1128/mSphere.00911-20.

Backbone

• Vector backbone: pAtetO
• Backbone manufacturer: modified from pASK-IBA37plus (IBA GmbH)
• Modifications to backbone: Removal of 6xHis tag, multiple cloning site, and Rop gene from pASK-IBA37plus
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bst-LF
• Alt name: Large-fragment Bst DNAP
• Alt name: Bst(exo-)
• Species: Geobacillus stearothermophilus
• Insert Size (bp): 1827
• Promoter: tet PA
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TACAGACCCTAATTTCACATCATATGAC
• 3′ sequencing primer: CCGACGAACTAAAACGCTTGAGGAC

Resource Information

• Supplemental Documents:
  • pAtetO_6xHis-Bst-LF.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.04.13.039941v3 for bioRxiv preprint.