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Addgene

pAG425 GAL Pong TPase L418A, L420A
(Plasmid #145795)

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Item Catalog # Description Quantity Price (USD)
Plasmid 145795 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAG415 GAL
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pong TPase L418A, L420A
  • Species
    Oryza Sativa
  • Mutation
    L418A, L420A

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG425 GAL Pong TPase L418A, L420A was a gift from Charles Nathan Hancock & Susan Wessler (Addgene plasmid # 145795 ; http://n2t.net/addgene:145795 ; RRID:Addgene_145795)

  • For your References section:

    Transposition of the Tourist-MITE mPing in yeast: an assay that retains key features of catalysis by the class 2 PIF/Harbinger superfamily. Hancock CN, Zhang F, Wessler SR. Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186/1759-8753-1-5. 10.1186/1759-8753-1-5 PubMed 20226077
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