pAG413 GAL ORF1 Shuffle1 NLS
(Plasmid
#145794)
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PurposemPing Yeast Transposition Assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 145794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG413 GAL
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORF1 Shuffle1 NLS
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SpeciesOryza Sativa
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MutationEnhanced Nucleur Localization Signal
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG413 GAL ORF1 Shuffle1 NLS was a gift from Charles Nathan Hancock (Addgene plasmid # 145794 ; http://n2t.net/addgene:145794 ; RRID:Addgene_145794) -
For your References section:
Alteration of the Ping and Pong ORF1 proteins allows for hyperactive transposition of mPing. Payero L, Outten G, Burckhalter CE, Hancock, CN. Journal of the South Carolina Academy of Science 2016, 14 (2):1-6.