pWL89A 4xE
(Plasmid
#145793)
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PurposemPing Yeast Transposition Assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 145793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWL89A
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4XE
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SpeciesOryza Sativa
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MutationInsertion of 4 copies of the CMV Enhancer
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWL89A 4xE was a gift from Charles Nathan Hancock (Addgene plasmid # 145793 ; http://n2t.net/addgene:145793 ; RRID:Addgene_145793) -
For your References section:
Development of mPing-based activation tags for crop insertional mutagenesis. Johnson A, Mcassey E, Diaz S, Reagin J, Redd PS, Parrilla DR, Nguyen H, Stec A, McDaniel LAL, Clemente TE, Stupar RM, Parrott WA, Hancock CN. Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. eCollection 2021 Jan. 10.1002/pld3.300 PubMed 33506165