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Addgene

pWL89A 2xE
(Plasmid #145792)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 145792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWL89A
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2xE
  • Species
    Oryza Sativa
  • Mutation
    Insertion of 2 copies of the CMV Enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWL89A 2xE was a gift from Charles Nathan Hancock (Addgene plasmid # 145792 ; http://n2t.net/addgene:145792 ; RRID:Addgene_145792)

  • For your References section:

    Development of mPing-based activation tags for crop insertional mutagenesis. Johnson A, Mcassey E, Diaz S, Reagin J, Redd PS, Parrilla DR, Nguyen H, Stec A, McDaniel LAL, Clemente TE, Stupar RM, Parrott WA, Hancock CN. Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. eCollection 2021 Jan. 10.1002/pld3.300 PubMed 33506165
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