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Addgene

PA1024_pET21a(+)C-tag
(Plasmid #14579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Merck
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    2-nitropropane dioxygenase
  • Alt name
    PA1024
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    987
  • GenBank ID
    NP_249715
  • Entrez Gene
    PA1024
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA1024_pET21a(+)C-tag was a gift from SeWon Suh (Addgene plasmid # 14579 ; http://n2t.net/addgene:14579 ; RRID:Addgene_14579)

  • For your References section:

    Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base. Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW. J Biol Chem. 2006 Jul 7. 281(27):18660-7. 10.1074/jbc.M601658200 PubMed 16682407
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