pCMV14-3X-Flag-SARS-CoV-2 S
(Plasmid #145780)

• Purpose: Over expression of SARS-CoV-2 S (spike) in mammalian cell line
• Depositing Lab: Zhaohui Qian
• Publication: Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi: 10.1038/s41467-020-15562-9.

Backbone

• Vector backbone: pCMV14-3×Flag
• Backbone size w/o insert (bp): 6310
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SARS-CoV-2 S
• Species: SARS CoV 2
• Insert Size (bp): 3819
• Mutation: Codon optimized and C-terminal 19 amino acids deletion
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFlag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pCMV14-3×Flag-SARS-CoV-2 S.gb
• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA