pCDNA3.1 hSCN5AdeltaSIV GFP Nter
(Plasmid #145770)

• Purpose: expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells
• Depositing Lab: Hugues Abriel
• Publication: Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi: 10.1161/CIRCRESAHA.110.228312. Epub 2010 Dec 16.

Backbone

• Vector backbone: pCDNA3.1-Zeo (+)
• Backbone size w/o insert (bp): 5015
• Total vector size (bp): 12015
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sodium voltage-gated channel alpha subunit 5
• Alt name: Nav1.5
• Alt name: SCN5A
• Species: H. sapiens (human)
• Insert Size (bp): 7000
• Mutation: Stop codon at Serine 2013
• GenBank ID:
• Entrez Gene: SCN5A (a.k.a. CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned GFP, a gift from T.Zimmer (University of Jenna, Germany), into plasmid pCDNA3.1 hSCN5a deltaSIV (ID: )

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry