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pcDNA3.1-kappa-myc-dK-2xG4S-TMst
(Plasmid #145766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 145766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5358
  • Total vector size (bp) 6459
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kappa-myc-dK-2xG4S-TMst
  • Alt name
    dK-TM
  • Alt name
    dl5(E52K)-TM
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1101
  • Mutation
    The dK FAP is dL5 with mutations at E50K, L89S, also known as E52K/L91S and NP138
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

http://pathways.mbic.cmu.edu/

For more information about this plasmid, please see the associated publication (PMID: 32786268).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-kappa-myc-dK-2xG4S-TMst was a gift from Marcel Bruchez (Addgene plasmid # 145766 ; http://n2t.net/addgene:145766 ; RRID:Addgene_145766)

  • For your References section:

    Protein Proximity Observed Using Fluorogen Activating Protein and Dye Activated by Proximal Anchoring (FAP-DAPA) System. Carpenter MA, Wang Y, Telmer CA, Schmidt BF, Yang Z, Bruchez MP. ACS Chem Biol. 2020 Aug 17. doi: 10.1021/acschembio.0c00419. 10.1021/acschembio.0c00419 PubMed 32786268
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