JP1520 PIK3CA E545K HA
(Plasmid #14571)

• Depositing Lab: Joan Brugge
• Publication: Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-1000.

Backbone

• Vector backbone: JP1520
• Backbone manufacturer: Jeff Engelman
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3CA E545K
• Alt name: PIK3CA
• Alt name: p110 alpha
• Species: B. taurus (bovine)
• Mutation: E545K
• Entrez Gene: PIK3CA
• Tag / Fusion Protein:
  • HA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Hemagglutinin (HA)–tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).