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Addgene

pGBW-m4046858
(Plasmid #145658)

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Item Catalog # Description Quantity Price (USD)
Plasmid 145658 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    ori-013 - marker | cmR
  • Backbone size w/o insert (bp) 1462
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 2'-O-ribose methyltransferase
  • Species
    Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    893
  • Mutation
    Escherichia coli recode 1
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ; Start Codon | ATG
  • Tag / Fusion Protein
    • Cleavable TEV;HA (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI Reference Sequence: YP_009725311.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4046858 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 145658 ; http://n2t.net/addgene:145658 ; RRID:Addgene_145658)
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