pGBW-m4046205
(Plasmid
#145470)
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PurposeYeast Expression plasmid for SARS-CoV-2 endoRNAse
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 145470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backboneAmpR; 2u URA3
- Backbone size w/o insert (bp) 4078
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 endoRNAse
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SpeciesS. cerevisiae (budding yeast); Severe acute respiratory syndrome coronavirus 2
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Insert Size (bp)1031
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Mutationwt
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter pTEF1
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Tag
/ Fusion Protein
- Cleavable thrombin;6xHIS (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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A portion of this plasmid was derived from a plasmid made byGinkgoBioworks
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NCBI Reference Sequence: YP_009725310.1Created by gene synthesis with Not1 site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBW-m4046205 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 145470 ; http://n2t.net/addgene:145470 ; RRID:Addgene_145470)