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Addgene

pGBW-m4046351
(Plasmid #145449)

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Item Catalog # Description Quantity Price (USD)
Plasmid 145449 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    AmpR; 2u URA3
  • Backbone size w/o insert (bp) 4078
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 2'-O-ribose methyltransferase
  • Species
    S. cerevisiae (budding yeast); Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    893
  • Mutation
    Saccharomyces cerevisiae recode 1
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter pGAL1
  • Tag / Fusion Protein
    • Cleavable thrombin;6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GinkgoBioworks

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI Reference Sequence: YP_009725311.1Created by gene synthesis with Not1 site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBW-m4046351 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 145449 ; http://n2t.net/addgene:145449 ; RRID:Addgene_145449)
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