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Addgene

pLV.PARP1#1
(Plasmid #14544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRDI292
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PARP1 shRNA
  • Alt name
    PARP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer H1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sense oligo sequence used for cloning: PARP-1 #1, 5'-GATCCCCGCT CTATCGAGTC GAGTACTTCA AGAGAGTACT CGACTCGATA GAGCTTTTTG GAAA-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV.PARP1#1 was a gift from Didier Trono (Addgene plasmid # 14544 ; http://n2t.net/addgene:14544 ; RRID:Addgene_14544)

  • For your References section:

    DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi Y, Turelli P, Masutani M, Trono D. J Virol. 2005 Mar . 79(5):2973-8. 10.1128/JVI.79.5.2973-2978.2005 PubMed 15709017
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