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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRDI292
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATR shRNA
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Alt nameATR
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SpeciesH. sapiens (human)
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Insert Size (bp)60
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Entrez GeneATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sense oligo sequence used for cloning: ATR, 5'-GATCCCCGGC GTCGTCTCAG CTCGTCTTCA AGAGAGACGA GCTGAGACGA CGCCTTTTTG GAAA-3'.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV.ATRi was a gift from Didier Trono (Addgene plasmid # 14543 ; http://n2t.net/addgene:14543 ; RRID:Addgene_14543) -
For your References section:
DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integration. Ariumi Y, Turelli P, Masutani M, Trono D. J Virol. 2005 Mar . 79(5):2973-8. 10.1128/JVI.79.5.2973-2978.2005 PubMed 15709017
