pCDNA3 Flag MKK7a1 (K76A)
(Plasmid #14541)

• Depositing Lab: Roger Davis
• Publication: Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7337-42.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK7a1(K76A)
• Alt name: MKK7
• Species: M. musculus (mouse)
• Insert Size (bp): 1080
• Mutation: Lys-76Ala
• Entrez Gene: Map2k7 (a.k.a. 5930412N11Rik, JNKK 2, Jnkk, Jnkk2, MAPKK 7, MEK 7, Mapkk7, Mek7, Mkk7, Prkm, Prkmk7, sek, sek2)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry