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Addgene

pET28b-6His-AcpS
(Plasmid #145376)

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Item Catalog # Description Quantity Price (USD)
Plasmid 145376 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AcpS
  • Species
    E. coli
  • Insert Size (bp)
    381
  • Entrez Gene
    acpP (a.k.a. b1094, ECK1080)
  • Tag / Fusion Protein
    • 6-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-6His-AcpS was a gift from Robert Tomko (Addgene plasmid # 145376 ; http://n2t.net/addgene:145376 ; RRID:Addgene_145376)

  • For your References section:

    A suite of PCR-based peptide tagging plasmids to permit epitope-targeted enzymatic functionalization of yeast proteins. Nemec AA, Tomko RJ Jr. Yeast. 2020 May 13. doi: 10.1002/yea.3471. 10.1002/yea.3471 PubMed 32401365
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