pmCellFree_KA2
(Plasmid
#145370)
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Purpose(Empty Backbone) Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 145370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmCellFree_KA2
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- His and mCherry (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- His and mCherry (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCellFree_KA2 was a gift from Rob Parton (Addgene plasmid # 145370 ; http://n2t.net/addgene:145370 ; RRID:Addgene_145370) -
For your References section:
Mapping Interactions among Cell-Free Expressed Zika Virus Proteins. Varasteh Moradi S, Gagoski D, Mureev S, Walden P, McMahon KA, Parton RG, Johnston WA, Alexandrov K. J Proteome Res. 2020 Apr 3;19(4):1522-1532. doi: 10.1021/acs.jproteome.9b00771. Epub 2020 Mar 9. 10.1021/acs.jproteome.9b00771 PubMed 32081002
