V250 pCEP-4HA B56epsilon
(Plasmid #14537)

• Depositing Lab: David Virshup
• Publication: Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Backbone

• Vector backbone: pCEP4 (modified)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10186
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPP2R5C B56 epsilon regulatory subunit of PP2A
• Alt name: B' epsilon
• Alt name: PR61 epsilon
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: L76703
• Entrez Gene: PPP2R5E (a.k.a. B56E, B56epsilon)
• Tag / Fusion Protein:
  • 4 HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: pCEP forward (invitrogen)
• 3′ sequencing primer: EBV Reverse (invitrogen)

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.