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Addgene

pmCellFree_KA1
(Plasmid #145369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 145369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCellFree_KA2
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • His and eGFP (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • His and eGFP (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCellFree_KA1 was a gift from Rob Parton (Addgene plasmid # 145369 ; http://n2t.net/addgene:145369 ; RRID:Addgene_145369)

  • For your References section:

    Mapping Interactions among Cell-Free Expressed Zika Virus Proteins. Varasteh Moradi S, Gagoski D, Mureev S, Walden P, McMahon KA, Parton RG, Johnston WA, Alexandrov K. J Proteome Res. 2020 Apr 3;19(4):1522-1532. doi: 10.1021/acs.jproteome.9b00771. Epub 2020 Mar 9. 10.1021/acs.jproteome.9b00771 PubMed 32081002
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