V249 pCEP-4HA B56delta
(Plasmid #14536)

• Depositing Lab: David Virshup
• Publication: Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Backbone

• Vector backbone: pCEP4 (modified)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10186
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPP2R5D B56 delta regulatory subunit of PP2A
• Alt name: B' delta
• Alt name: PR61 delta, B56D
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Mutation: R210H
• GenBank ID: L76702
• Entrez Gene: PPP2R5D (a.k.a. B56D, B56delta, HJS1, MRD35)
• Tag / Fusion Protein:
  • 4 HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: pCEP forward (invitrogen)
• 3′ sequencing primer: EBV Reverse (invitrogen)

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Please note that the Addgene verified sequence finds an R210H mutation in PPP2R5D. Other differences compared to the depositor sequence do not affect plasmid function.