V248 pCEP 4HA B56gamma3
(Plasmid #14535)

• Depositing Lab: David Virshup
• Publication: Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Backbone

• Vector backbone: pCEP4 (modified)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10186
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPP2R5C B56 gamma3 regulatory subunit of PP2A
• Alt name: B' gamma3
• Alt name: PR61 gamma3
• Alt name: PPP2R5C
• Species: H. sapiens (human)
• Insert Size (bp): 1900
• GenBank ID: L42374
• Entrez Gene: PPP2R5C (a.k.a. B56G, B56gamma, PR61G)
• Tag / Fusion Protein:
  • 4 HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pCEP forward (invitrogen)
• 3′ sequencing primer: EBV Reverse (invitrogen)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Mumby, UTSW
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.