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Addgene

pmCellFree_KA1_ZIKV NS3
(Plasmid #145345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 145345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCellFree_KA1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZIKV NS3
  • Species
    NC_012532 (ZIKAV)
  • Promoter T7- CMV/CAG
  • Tag / Fusion Protein
    • 8xHis and eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCellFree_KA1_ZIKV NS3 was a gift from Rob Parton (Addgene plasmid # 145345 ; http://n2t.net/addgene:145345 ; RRID:Addgene_145345)

  • For your References section:

    Mapping Interactions among Cell-Free Expressed Zika Virus Proteins. Varasteh Moradi S, Gagoski D, Mureev S, Walden P, McMahon KA, Parton RG, Johnston WA, Alexandrov K. J Proteome Res. 2020 Apr 3;19(4):1522-1532. doi: 10.1021/acs.jproteome.9b00771. Epub 2020 Mar 9. 10.1021/acs.jproteome.9b00771 PubMed 32081002
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