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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEP4 (modified)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10186
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPP2R5A B56 alpha regulatory subunit of PP2A
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Alt nameB' alpha
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Alt namePR61 alpha
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SpeciesH. sapiens (human)
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Insert Size (bp)2900
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GenBank IDL42373
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Entrez GenePPP2R5A (a.k.a. B56A, B56alpha, PR61A)
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Tag
/ Fusion Protein
- 4 HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pCEP forward (invitrogen)
- 3′ sequencing primer EBV Reverse (invitrogen) (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V245 pCEP-4HA B56alpha was a gift from David Virshup (Addgene plasmid # 14532 ; http://n2t.net/addgene:14532 ; RRID:Addgene_14532) -
For your References section:
Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A. Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM. Science. 1999 Mar 26. 283(5410):2089-91. 10.1126/science.283.5410.2089 PubMed 10092233