pcDNA3-sACE2v3-sfGFP
(Plasmid #145174)

• Purpose: Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3
• Depositing Lab: Erik Procko
• Publication: Chan et al Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 7939
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Angiotensin-converting enzyme 2
• Alt name: ACE2
• Alt name: peptidyl-dipeptidase A
• Species: H. sapiens (human)
• Insert Size (bp): 2607
• Mutation: Extracellular, soluble protease domain (a.a. M1-D615) with mutations A25V, T27Y, T92Q, Q325P, A386L
• GenBank ID: NM_021804.1
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Promoter: CMV
• Tag / Fusion Protein:
  • Linker GSGGSGSGG and superfolder GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.