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pJT175_GalL_A3A(Y130F)Δ186-BE3
(Plasmid #145124)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 145124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p415
  • Backbone manufacturer
    p415-GalL-Cas9-CYC1t (Plasmid #43804)
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3A(Y130F)Δ186-BE3
  • Alt name
    A3A(Y130F;1-186AA)-nCas9-UGI-NLS
  • Species
    H. sapiens (human); Bacillus phage PBS2, Streptococcus pyogenes
  • Insert Size (bp)
    4959
  • Mutation
    A3A(Y130F; 1-186AA); spCas9(D10A)
  • Promoter GalL

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT175_GalL_A3A(Y130F)Δ186-BE3 was a gift from Ralph Bock (Addgene plasmid # 145124 ; http://n2t.net/addgene:145124 ; RRID:Addgene_145124)

  • For your References section:

    Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Tan J, Zhang F, Karcher D, Bock R. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. 10.1038/s41467-020-14465-z PubMed 32005820
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