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pJT131_GalL_cCDA1-NGBE3
(Plasmid #145095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 145095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p415
  • Backbone manufacturer
    p415-GalL-Cas9-CYC1t (Plasmid #43804)
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cCDA1-NGBE3
  • Alt name
    nCas9-NG-XTEN-pmCDA1-UGI-NLS
  • Species
    Bacillus phage PBS2, Petromyzon marinus, Streptococcus pyogenes
  • Insert Size (bp)
    5073
  • Mutation
    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
  • Promoter GalL

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Based on the plasmid pRS315e_pGal-nCas9(D10A)-PmCDA1(Akihiko Kondo, Addgene plasmid # 79617)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT131_GalL_cCDA1-NGBE3 was a gift from Ralph Bock (Addgene plasmid # 145095 ; http://n2t.net/addgene:145095 ; RRID:Addgene_145095)

  • For your References section:

    Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Tan J, Zhang F, Karcher D, Bock R. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. 10.1038/s41467-020-14465-z PubMed 32005820
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