pJT43_GalL_BE-P(AP)3
(Plasmid
#145036)
-
PurposeExpresses BE-P(AP)3 in yeast cells
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 145036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonep415
-
Backbone manufacturerp415-GalL-Cas9-CYC1t (Plasmid #43804)
-
Vector typeYeast Expression, CRISPR
-
Selectable markersLEU2
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBE-(AP)3
-
Alt namerAPOBEC1-(AP)3-Cas9n-UGI-NLS
-
SpeciesBacillus phage PBS2, Rattus norvegicus(rat), Streptococcus pyogenes
-
Insert Size (bp)5109
-
MutationspCas9(D10A)
- Promoter GalL
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T3 promoter
- 3′ sequencing primer T7 promoter (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJT43_GalL_BE-P(AP)3 was a gift from Ralph Bock (Addgene plasmid # 145036 ; http://n2t.net/addgene:145036 ; RRID:Addgene_145036) -
For your References section:
Engineering of high-precision base editors for site-specific single nucleotide replacement. Tan J, Zhang F, Karcher D, Bock R. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. 10.1038/s41467-018-08034-8 PubMed 30683865
