pcDNA3.1-hACE2
(Plasmid #145033)

• Purpose: Express human ACE2 with C9 tag at C-terminal in mammalian cells
• Depositing Lab: Fang Li
• Publication: Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 10.1038/s41586-020-2179-y.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 2450
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human ACE2
• Species: H. sapiens (human)
• Insert Size (bp): 2445
• Entrez Gene: ACE2 (a.k.a. ACEH)
• Promoter: CMV
• Tag / Fusion Protein:
  • C9 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: none (not destroyed)
• 5′ sequencing primer: T7-F
• 3′ sequencing primer: BGH-R

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA