pcDNA3.1-SARS-Spike
(Plasmid #145031)

• Purpose: Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells
• Depositing Lab: Fang Li
• Publication: Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586-020-2179-y. doi: 10.1038/s41586-020-2179-y.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV Spike
• Species: Synthetic
• Insert Size (bp): 3840
• Promoter: CMV
• Tags / Fusion Proteins:
  • C9 (C terminal on insert)
  • CD5 signal peptide (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7-F
• 3′ sequencing primer: BGH-R

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please note that immediate availability of this material is restricted to U.S. customers only. For international customers, an export license is required. If you are interested in obtaining this plasmid, please contact