pPB-3xnls-Tq-Ca-FLITS
(Plasmid #145030)

• Purpose: piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes
• Depositing Lab: Dorus Gadella
• Publication: van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.

Backbone

• Vector backbone: piggyBac
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xnls-Tq-Ca-FLITS
• Alt name: mTurquoise calcium biosensor
• Species: Synthetic
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xnls-Tq-Ca-FLITS

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • 5256_pPuro-PB_3xNLS_mTq2_calcium_lifetime_sense.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint at bioRxiv: doi.org/10.1101/2021.06.21.449214