pET_RTX(exo-)-6xHis
(Plasmid #145028)

• Purpose: pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
• Depositing Lab: Andrew Ellington
• Publication: Bhadra et al BioProtocol 2021

Backbone

• Vector backbone: modified pET21
• Backbone size w/o insert (bp): 5871
• Total vector size (bp): 8232
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RTX(exo-)
• Alt name: Exonuclease deficient variant of RTX
• Species: Synthetic
• Insert Size (bp): 2361
• Mutation: N210D
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GATCTCGATCCCGCGAAATTAATACGAC
• 3′ sequencing primer: TTGCTCAGCGGTGGCAGCAGC

Resource Information

• Supplemental Documents:
  • pET_RTX(exo-)-6xHis
• A portion of this plasmid was derived from a plasmid made by: Jared Ellefson (Ellington Lab)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2020.03.29.013342 for bioRxiv preprint.