pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS
(Plasmid #145027)

• Purpose: Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker
• Depositing Lab: Dorus Gadella
• Publication: van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.

Backbone

• Vector backbone: pLV
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xnls-Tq-Ca-FLITS
• Alt name: mTurquoise calcium biosensor
• Species: Synthetic
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xnls-Tq-Ca-FLITS (C terminal on backbone)
  • H2B-Maroon (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint at bioRxiv: doi.org/10.1101/2021.06.21.449214