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Addgene

pRSET FLIPmal
(Plasmid #14478)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14478 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRSET
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPmal
  • Alt name
    FLIPmalE WT
  • Alt name
    MBP
  • Species
    E. coli
  • Insert Size (bp)
    2700
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

A truncated malE PCR product encoding mature maltose-binding protein (MBP) without N-terminal signal peptide (position 79–1188 relative to the ATG) was fused between the two GFP genes. Subsequently, the chimeric fragment was inserted into pRSET.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLIPmal was a gift from Wolf Frommer (Addgene plasmid # 14478 ; http://n2t.net/addgene:14478 ; RRID:Addgene_14478)
  • For your References section:

    Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Fehr M, Frommer WB, Lalonde S. Proc Natl Acad Sci U S A. 2002 Jul 23. 99(15):9846-51. 10.1073/pnas.142089199 PubMed 12097642