pRSET FLIPmal
(Plasmid #14478)

• Depositing Lab: Wolf Frommer
• Publication: Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 99(15):9846-51.

Backbone

• Vector backbone: pRSET
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FLIPmal
• Alt name: FLIPmalE WT
• Alt name: MBP
• Species: E. coli
• Insert Size (bp): 2700
• Tags / Fusion Proteins:
  • ECFP (N terminal on insert)
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

A truncated malE PCR product encoding mature maltose-binding protein (MBP) without N-terminal signal peptide (position 79–1188 relative to the ATG) was fused between the two GFP genes. Subsequently, the chimeric fragment was inserted into pRSET.