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p4949 pLPCX-His-Xpress NLS hBrd4 CTD
(Plasmid #14459)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPCX
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brd4 CTD
  • Alt name
    Brd4
  • Alt name
    hBrd4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    amino acids 1047-1362 of Brd4 (the CTD). Functions in a dominant-negative manner by competing with endgogenous Brd4 for binding to E2.
  • Entrez Gene
    BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer LNCX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The His-Xpress-NLS-hBrd4-CTD cassette was reamplified by PCR and subcloned into pLPCX plasmid (BD Biosciences) to generate the pLPCX-His-Xpress-NLS-hBrd4-CTD plasmid for stable cell line construction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4949 pLPCX-His-Xpress NLS hBrd4 CTD was a gift from Peter Howley (Addgene plasmid # 14459 ; http://n2t.net/addgene:14459 ; RRID:Addgene_14459)

  • For your References section:

    Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Cell. 2004 Apr 30. 117(3):349-60. 10.1016/S0092-8674(04)00402-7 PubMed 15109495
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