p5071 pcDNA4c hBrd4 (595-1362)
(Plasmid
#14450)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4 / HisMax C
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBrd4 (595-1362)
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Alt nameBrd4
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Alt namehBrd4
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SpeciesH. sapiens (human)
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Insert Size (bp)2300
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Mutationamino acids 595-1362
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Entrez GeneBRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5071 pcDNA4c hBrd4 (595-1362) was a gift from Peter Howley (Addgene plasmid # 14450 ; http://n2t.net/addgene:14450 ; RRID:Addgene_14450) -
For your References section:
Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Cell. 2004 Apr 30. 117(3):349-60. 10.1016/S0092-8674(04)00402-7 PubMed 15109495
Map uploaded by the depositor.
