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Addgene

p5069 pcDNA4c hBrd4 (471-730)
(Plasmid #14448)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4 / HisMax C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brd4 (471-730)
  • Alt name
    Brd4
  • Alt name
    hBrd4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • Mutation
    amino acids 471-730
  • Entrez Gene
    BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5069 pcDNA4c hBrd4 (471-730) was a gift from Peter Howley (Addgene plasmid # 14448 ; http://n2t.net/addgene:14448 ; RRID:Addgene_14448)

  • For your References section:

    Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Cell. 2004 Apr 30. 117(3):349-60. 10.1016/S0092-8674(04)00402-7 PubMed 15109495
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