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Addgene

p5068 pGEX-6P-1 Brd4 full-length
(Plasmid #14447)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14447 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brd4
  • Alt name
    Brd 4
  • Alt name
    hBrd4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4100
  • Entrez Gene
    BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5068 pGEX-6P-1 Brd4 full-length was a gift from Peter Howley (Addgene plasmid # 14447 ; http://n2t.net/addgene:14447 ; RRID:Addgene_14447)
  • For your References section:

    Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Cell. 2004 Apr 30. 117(3):349-60. 10.1016/S0092-8674(04)00402-7 PubMed 15109495