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Addgene

p5067 pOZN E2TR
(Plasmid #14446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOZ-N
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    IL-2 receptor

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E2TR
  • Alt name
    E2 transcriptional repressor
  • Species
    bovine papillomavirus type 1 (BPV-1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pBMN5 (5'-GCTTGGATACACGCCGC-3')
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5067 pOZN E2TR was a gift from Peter Howley (Addgene plasmid # 14446 ; http://n2t.net/addgene:14446 ; RRID:Addgene_14446)

  • For your References section:

    Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Cell. 2004 Apr 30. 117(3):349-60. 10.1016/S0092-8674(04)00402-7 PubMed 15109495
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