p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)
(Plasmid #14443)

• Depositing Lab: Peter Howley
• Publication: You et al Cell. 2004 Apr 30. 117(3):349-60.

Backbone

• Vector backbone: pcDNA3.1 / V5-His-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brd4 (1-461)
• Alt name: Brd4
• Alt name: hBrd4
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: amino acids 1-461
• Entrez Gene: BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: TOPO TA cloning (unknown if destroyed)
• 3′ cloning site: TOPO TA cloning (unknown if destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fragments of human Brd4 cDNA were cloned into pcDNA3.1/V5-His using PCR and TOPO TA Expression Kit (Invitrogen) for expression controlled by both a CMV promoter and a T7 promoter. These plasmids were used for in vitro transcription and translation.