p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD
(Plasmid #14442)

• Depositing Lab: Peter Howley
• Publication: You et al Cell. 2004 Apr 30. 117(3):349-60.

Backbone

• Vector backbone: pcDNA4 / HisMax C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brd4 CTD
• Alt name: Brd4
• Alt name: hBrd4
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: amino acids 1047-1362 of Brd4 (the CTD). Functions in a dominant-negative manner by competing with endgogenous Brd4 for binding to E2.
• Entrez Gene: BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
• Tag / Fusion Protein:
  • SV40 NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To construct an expression plasmid for the Brd4-CTD, the SV40 nuclear localization signal (NLS) was inserted in frame in front of the Brd4-CTD coding region by PCR and the product was subcloned into pcDNA4C vector using BamHI and NotI sites.