p4947 pcDNA4c hBrd4 full-length
(Plasmid #14441)

• Depositing Lab: Peter Howley
• Publication: You et al Cell. 2004 Apr 30. 117(3):349-60.

Backbone

• Vector backbone: pcDNA4 / HisMax C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brd4
• Alt name: Brd 4
• Alt name: hBrd4
• Species: H. sapiens (human)
• Insert Size (bp): 4100
• Entrez Gene: BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • T7 tag (N terminal on backbone)
  • Xpress epitope (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results find amino acid change T249P compared to hBrd4 reference sequence NP_490597.1