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Addgene

p3259 pCMV hUBC9 mt HA Notes

Addgene Diagnostic Digest

The following gel shows the NEB 1kb ladder, uncut plasmid, the plasmid cut with BglII and the plasmid cut with EcoRI.

We see the expected pattern with EcoRI (~3.6kb and ~1.6kb).

The BglII enzyme was found to be expired.