p3259 pCMV hUBC9 mt HA
(Plasmid #14439)

• Depositing Lab: Peter Howley
• Publication: Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):2005-10.

Backbone

• Vector backbone: pCMV4
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBC9 C93S
• Alt name: UBC 9
• Alt name: hUBC9
• Species: H. sapiens (human)
• Insert Size (bp): 500
• Mutation: Cys93Ser mutation (TGC changed to AGC)
• Entrez Gene: UBE2I (a.k.a. C358B7.1, P18, UBC9)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BclI/BglII (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A fragment containing the full coding region of hubc9 C93S cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3′-end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.